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mcherry激发波长是多少?

作者:时间:2019-09-05 10:26浏览14861 次

信息摘要:

mCherry是一种来自于蘑菇珊瑚(mushroom coral)的红色荧光蛋白,常有于标记和示踪某些分子和细胞组分。相对于其他荧光,mCherry的好处在于它的颜色和应用最多的绿色荧光蛋白(GFP)能进行共同标记,并且mCherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry激发波长是多少?

mCherry是一种来自于蘑菇珊瑚(mushroom coral)的红色荧光蛋白,常有于标记和示踪某些分子和细胞组分。相对于其他荧光,mcherry的好处在于它的颜色和应用最多的绿色荧光蛋白(GFP)能进行共同标记,并且mcherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry红色荧光蛋白
吸收和发射波长:

mcherry的更大吸收/发射峰分别位于587nm和610nm,对光致漂白耐受,荧光非常稳定。

mcherry红色荧光蛋白

常见应用:
•mCherry常用于与目的基因组成融合蛋白以及通过IRES或2A与感兴趣的蛋白共表达;
•启动子活性研究;
•荧光共振能量转移(fluorescence resonance energy transfer,FRET)和其他定量实验;
•标记细胞或者分子,进行示踪实验;

mcherry红色荧光蛋白

 mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

 
m前缀的意义:
mCherry的m为monmer单体的缩写,表示mCherry荧光蛋白的形式为单体,这在很多实验设计中非常重要,比如与目的标记基因组成融合蛋白时。

成熟时间:
mCherry具有较快的成熟速度,t0.5为15分钟,这在一些需要做出快速反应的实验非常重要。比如启动子活性报告系统等。

如何观察和筛选mcherry在植物中的表达?

你可以选用LUYOR-3415RG和LUYOR-3260GR便携式荧光蛋白激发光源来直接观察和筛选mcherry在植物中有没有表达。如需进一步了解,请拨打电话153-1756-5658进行咨询(或直接添加微信15317565658咨询)。

mcherry红色荧光蛋白

mcherry红色荧光蛋白的激发波长和发射波长

1573530098248906.jpg

上图为LUYOR-3415RG便携式红色荧光蛋白激发光源

1573530166208008.jpg

上图为LUV-50A红色荧光蛋白观察眼镜

1634021167907968.jpg

上图为LUV-590A 红色荧光蛋白拍照滤镜

1638151326207096.jpg

上图为红色荧光蛋白在大豆根系上的表达(LUYOR-3415RG照射,LUV-590A滤镜拍摄)


实验室常见荧光蛋白的激发波长和发射波长

紫外荧光蛋白UV Proteins

Protein Excitation Wavelength   Emission Wavelength       
Sirius 355 424
Sandercyanin 375 630
shBFP-N158S/L173I 375 458

蓝色荧光蛋白Blue Proteins

Protein Excitation Wavelength Emission Wavelength
Azurite 383 450
EBFP2 383 448
mKalama1 385 456
mTagBFP2                        399 454
TagBFP 402 457
shBFP 401 458

青色荧光蛋白Cyan Proteins

Protein Excitation Wavelength Emission Wavelength
ECFP 433 475
Cerulean 433 475
mCerulean3 433 475
SCFP3A 433 474
CyPet 435 477
mTurquoise 434 474
mTurquoise2 434 474
TagCFP 458 480
mTFP1 462 492
monomeric Midoriishi-Cyan 470 496
Aquamarine 430 474

绿色荧光蛋白Green Proteins

Protein Excitation Wavelength Emission Wavelength
TurboGFP 482 502
TagGFP2 483 506
mUKG 483 499
Superfolder GFP 485 510
Emerald 487 509
EGFP 488 507
Monomeric Azami Green    492 505
mWasabi 493 509
Clover 505 515
mNeonGreen 506 517
NowGFP 494 502
mClover3 506 518

黄色荧光蛋白Yellow Proteins

Protein Excitation Wavelength Emission Wavelength
TagYFP 508 524
EYFP 513 527
Topaz 514 527
Venus 515 528
SYFP2 515 527
Citrine 516 529
Ypet 517 530
lanRFP-ΔS83                       521 592
mPapaya1 530 541
mCyRFP1 528 594

桔色荧光蛋白Orange Proteins

Protein Excitation Wavelength Emission Wavelength
Monomeric Kusabira-Orange 548 559
mOrange 548 562
mOrange2 549 565
mKOκ 551 563
mKO2 551 565

红色荧光蛋白Red Proteins

Protein Excitation Wavelength Emission Wavelength
TagRFP 555 584
TagRFP-T 555 584
RRvT 556 583
mRuby 558 605
mRuby2 559 600
mTangerine                             568 585
mApple 568 592
mStrawberry 574 596
FusionRed 580 608
mCherry 587 610
mNectarine 558 578
mRuby3 558 592
mScarlet 569 594
mScarlet-I 569 593

远红荧光蛋白Far Red Proteins

Protein Excitation Wavelength Emission Wavelength
mKate2 588 633
HcRed-Tandem 590 637
mPlum 590 649
mRaspberry 598 625
mNeptune 600 650
NirFP 605 670
TagRFP657                             611 657
TagRFP675 598 675
mCardinal 604 659
mStable 597 633
mMaroon1 609 657
mGarnet2 598 671

近红荧光蛋白Near IR Proteins

Protein Excitation Wavelength Emission Wavelength
iFP1.4 684 708
iRFP713 (iRFP)                      690 713
iRFP670 643 670
iRFP682 663 682
iRFP702 673 702
iRFP720 702 720
iFP2.0 690 711
mIFP 683 704
TDsmURFP 642 670
miRFP670 642 670

Sapphire-type Proteins

Protein Excitation Wavelength Emission Wavelength
Sapphire 399 511
T-Sapphire                              399 511
mAmetrine 406

526

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.


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