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yfp激发光波长和YFP的发射波长是多少?

作者:时间:2019-09-05 10:33浏览5088 次

信息摘要:

黄色荧光蛋白(Yellow Fluorescent Protein ,YFP)可以看做绿色荧光蛋白的一种突变体,最初来源于维多利亚多管水母( Aequorea victoria)。相对于绿色荧光蛋白,其荧光向红色光谱偏移,而这主要是由于蛋白203位苏氨酸变为酪氨酸。其最大激发波长为514 nm,最大发射波长为527 nm。

YFP激发波长为510nm,最大发射波长为527 nm

黄色荧光蛋白(Yellow Fluorescent Protein ,YFP)可以看做GFP.html' target='_blank' title='绿色荧光蛋白' >绿色荧光蛋白的一种突变体,最初来源于维多利亚多管水母( Aequorea victoria)。相对于绿色荧光蛋白,其荧光向红色光谱偏移,而这主要是由于蛋白203位苏氨酸变为酪氨酸。其最大激发波长为514 nm,最大发射波长为527 nm。

黄色荧光蛋白

 

黄色荧光蛋白的激发波长

黄色荧光蛋白的发射波长

美国路阳生产有多款便携式激发光源,无需荧光显微镜就能轻松观察筛选黄色荧光蛋白表达阳性。LUYOR-3260CY便携式YFP激发光源能直接分选YFP表达阳性的植株,能够分选愈伤组织、植株、种子、菌落等。如欲进一步了解便携式荧光蛋白激发光源,可直接联系上海路阳生物技术有限公司的销售客服。

黄色荧光蛋白激发光源

Yellow Fluorescent Proteins/常用黄色荧光蛋白的激发波长和发射波长

YFP种类 激发光波长(nm) 发射光波长(nm)
EYFP 514 527
Topaz 514 527
Venus 515 528
mCitrine 516 529
YPet 517 530
TagYFP 508 524
PhiYFP 525 537
ZsYellow1 529 539
mBanana 540 553

 

Yellow Fluorescent Proteins

Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.

 黄色荧光蛋白的激发波长和发射波长

Fluorochrome Excitation Wavelength (Nanometers) Emission Wavelength (Nanometers)
Acridine Orange (Bound to DNA) 500 526
Alexa Fluor 488 495 519
Alexa Fluor 500 503 525
Aminofluorescein 497 519
Astrazon Orange R 470 540
Aurophosphine 450-490 515
BCECF (Biscarboxyethylcarboxyfluorescein) 505 530
BODIPY 492/515 (Difluoroboradiazaindacene) 490 515
BODIPY 493/503 500 506
BODIPY 500/510 509 515
BODIPY 505/515 502 510
BODIPY FL 503 512
Calcein 494 517
Calcium Green 506 533
CellTracker Green CMFDA (Chloromethylfluorescein Diacetate) 492 517
CFDA (Carboxyfluorescein Diacetate) 495 520
Chinacrine 450-495 515
Cl-NERF 514 (High pH) 540 (High pH)
CyQuant GR 480 520
DCFH (Carboxydichlorofluorescein) 505 535
DiBAC4(3) (Bisdibutylbarbituric Acid) 493 516
DiO (Dipentyloxacarbocyanine Iodide) 484 502
DiOC16(3) 484 501
DiOC18(3) 484 501
DiOC2(3) 482 497
DiOC5(3) 484 500
DiOC6(3) 484 511
DiOC7(3) 482 504
DM-NERF 510 (High pH) 497 (Low pH) 536 (High pH) 527 (Low pH)
DTAF (Fluorescein Dichlorotriazine) 494 520
FAM (Carboxyfluorescein) 492 518
FDA (Fluorescein Diacetate) 495 520
FITC (Fluorescein Isothiocyanate) 494 518
FlCRhR (Cyclic AMP Fluorosensor) 500 517
Fluo-3 480-506 520
Fluo-4 494 516
Fluo-4FF 494 518
Fluo-5F 494 516
Fluo-5N 493 518
Fluorescein 494 518
Fluorescein Sulfonic Acid 476 519
FluoroEmerald 495 524
FluorX 494 520
FluoZin-1 495 517
FluoZin-2 494 521
FluoZin-3 494 516
GFP-RS (Red-Shifted) 498 516
LysoTracker Green DND-26 504 511
Magnesium Green 506 531
MitoFluor Green 490 516
MitoTracker Green FM 490 516
NeuroTrace 500/525 500 525
NeuroTrace 515/535 515 535
Newport Green DCF 506 535
Newport Green PDX 490 518
Nonyl Acridine Orange 495 519
Oligreen 498 518
Oregon Green 488 496 524
Oregon Green 500 503 522
Oregon Green 514 511 530
PFB-F (Pentafluorobenzoyl Fluorescein) 492 516
Phen Green FL 492 517
Phen Green SK 507 532
Pico Green 502 523
PKH67 496 520
Rhodamine 110 496 520
Rhodamine 123 507 529
Rhodamine Green 502 527
Sodium Green 506 532
SpectrumGreen 1 497 524
SpectrumGreen 2 509 538
Stachyose Fluorescein 491 516
SYBR Gold 495 537
SYBR Green I 498 522
SYBR Green II 492 513
SYTO 11 508 527
SYTO 12 499 522
SYTO 13 488 509
SYTO 16 488 518
SYTO 21 494 517
SYTO 23 499 520
SYTO 24 490 515
SYTOX Green 504 523
TAMRA (Carboxytetramethylrhodamine) 504 529
YFP/EYFP (Enhanced Yellow Fluorescent Protein) 512 529
YOYO-1, YO-PRO-1 491 509

 


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